Anticancer activity of polyphenolic <i>Punica granatum</i> peel extracts obtained by hybrid ultrasound‐microwave assisted extraction: Evaluation on HeLa and HepG2 cells

Abstract Punica granatum peel is a significant source of polyphenolic compounds that have potential uses as drugs. This study aimed to obtain and purify derived from pomegranate evaluate their anticancer activity in cell lines. Polyphenols the were extracted using hybrid ultrasound‐microwave technology. Different mass:volume ratios water:ethanol used during extraction. Purification was performed on chromatographic column with Amberlite XAD‐16, unfractionated extracts fractions obtained. The main identified by HPLC belong ellagitannins family. extract 1:12 (m/v) 30% ethanol condition its cytotoxic effect against HeLa, HepG2, 3T3 MTT assay cytotoxicity (PPEs). Viability HeLa HepG2 cells affected dose‐dependent manner concentration 25 ppm polyphenol powders. Additionally, genotoxicity test carried out, which showed DNA damage, indicating possibly due part damage. use could be viable option for obtaining bioactive can adjuvants or treatment various diseases such cancer.